I need help I have a problem with refund an app (Nebo) I purchased is by a mistake but when I try to request a refund they tell me (We've determined that your purchase doesn't meet the conditions for a refund) please help me.

Purchases are considered final as you know, but, and depending how you've already contacted Support, you could try contacting them via : http://www.apple.com/support/itunes/ww (click on your country's flag, the country where you live, and which is therefore the country on the account ; click on Contact Support towards the top right ; click on Get Help under 'Talk To Us’)

My money has debited sudden in apple store i dont no how so kindly refund my money to my account urjnt

Am I able to get a refund of 65.31$ because I canceled my subscription with class dojo and they already took my money from my card

We can't say whether you can or can't (purchases are considered final), we are fellow users. Try contacting iTunes Support and see what they say.